Fig 2: Induced ILC2 depletion exacerbates post-MI cardiac dysfunction. (A) Mouse surgery and treatment strategy. (B/C) Representative FACS analysis of splenic ILC2 and EOS in ICOS-T mice treated with or without IL-33 for 3 days to increase ILC2 production, and then with or without DTX injection for 3 days as indicated. (D) Cardiac functions, HW/BW, and HW/TL in ICOS-T mice with different treatments (without IL33 treatment) at 28 days post-MI. (E) Infarct thickness and infarct size ratio. (F) FACS analysis of heart CD45+CD11b+Siglec-F+ EOS at 1 day post-MI (without IL33 treatment). (G) TUNEL detected apoptotic cells in hearts from different groups of mice at 28 days post-MI or sham surgery. Representative images are shown to the left. Scales: 1.5 mm (E) and 100 µm (G). Data are mean ± SEM. n = 8∼10 mice per sham group, n = 13∼15 mice per MI group, one-way ANOVA test followed by a post hoc Tukey’s test (D and F) or non-parametric Mann–Whitney U test followed by Bonferroni correction (E and G).